Immunogenicity in Macaques of the clinical product for a clade B DNA/MVA HIV vaccine:: Elicitation of IFN-γ, IL-2, and TNF-α coproducing CD4 and CD8 T cells

被引：27

作者：

Robinson, Harriet L. ^{[1
,2
]}

Sharma, Sunita ^{[1
,2
]}

Zhao, Jun ^{[1
,2
]}

Kannanganat, Sunil ^{[1
,2
]}

Lai, Lilin ^{[1
,2
]}

Chennareddi, Lakshmi ^{[1
,2
]}

Yu, Tianwei ^{[2
,3
]}

Montefiori, David C. ^{[4
]}

Amara, Rama Rao ^{[1
,2
]}

Wyatt, Linda S. ^{[5
]}

Moss, Bernard ^{[5
]}

机构：

[1] Emory Univ, Yerkes Natl Primate Res Ctr, Atlanta, GA 30329 USA

[2] Emory Vaccine Ctr, Atlanta, GA 30322 USA

[3] Emory Univ, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA

[4] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA

[5] NIAID, Natl Inst Hlth, Viral Dis Lab, Bethesda, MD 20892 USA

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 2007年 / 23卷 / 12期

关键词：

D O I：

10.1089/aid.2007.0165

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The clinical product for a clade B HIV DNA/MVA vaccine expressing Gag, Pol, and Env has been tested for immunogenicity in macaques. Responding T cells were at the threshold for detection following DNA priming at weeks 0 and 8 but underwent sharp expansions and contractions following MVA boosting at weeks 16 and 24. Both CD4 and CD8 T cell responses had high frequencies of cytokine coproducing cells with > 50% of the memory cells coproducing multiple cytokines including IL-2. The highest responses were elicited to Gag, followed by Env and then Pol. In two of six macaques, the vaccine also elicited low levels of neutralizing Ab for easy to neutralize clade B isolates.

引用

页码：1555 / 1561

页数：7

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