Allelotype analysis of oesophageal adenocarcinoma: loss of heterozygosity occurs at multiple sites

被引:69
作者
Dolan, K
Garde, J
Gosney, J
Sissons, M
Wright, T
Kingsnorth, AN
Walker, SJ
Sutton, R
Meltzer, SJ
Field, JK [1 ]
机构
[1] Univ Liverpool, Mol Genet & Oncol Grp, Liverpool L69 3BX, Merseyside, England
[2] Univ Liverpool, Dept Surg, Liverpool L69 3BX, Merseyside, England
[3] Univ Liverpool, Dept Pathol, Liverpool L69 3BX, Merseyside, England
[4] Blackpool Victoria Infirm, Dept Pathol, Blackpool FY3 8NR, England
[5] Blackpool Victoria Infirm, Dept Surg, Blackpool FY3 8NR, England
[6] Univ Maryland, Div Gastroenterol, Baltimore, MD 21201 USA
关键词
oesophageal adenocarcinoma; loss of heterozygosity; fractional allele loss;
D O I
10.1038/bjc.1998.607
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Deletions of tumour-suppressor genes can be detected by loss of heterozygosity (LOH) studies, which were performed on 23 cases of adenocarcinoma of the oesophagus, using 120 microsatellite primers covering ail non-acrocentric autosomal chromosome arms. The chromosomal arms most frequently demonstrating LOH were 3p (64% of tumours), 5q (45%), 9p (52%), 11p (61%), 13q (50%), 17p (96%), 17q (55%) and 18q (70%). LOH on 3p, 9p, 13q, 17p and 18q occurred mainly within the loci of the VHL, CDKN2, Rb, TP53 and DCC tumour-suppressor genes respectively. LOH on 5q occurred at the sites of the MSH3 mismatch repair gene and the APC tumour-suppressor gene. 11p15.5 and 17q25-qter represented areas of greatest LOH on chromosomes 11p and 17q, and are putative sites of novel tumour-suppressor genes. LOH on 9p was significantly associated with LOH on 59, and tumours demonstrating LOH at both the CDKN2 (9p21) and MSH3 (5q11-q12) genes had a significantly higher fractional allele loss than those retaining heterozygosity at these sites. Six of nine carcinomas displaying microsatellite alterations also demonstrated LOH at CDKN2, which may be associated with widespread genomic instability, Overall, there are nine sites of LOH associated with oesophageal adenocarcinoma.
引用
收藏
页码:950 / 957
页数:8
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