Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance

被引：108

作者：

Tabashnik, Bruce E. ^{[1
]}

Huang, Fangneng ^{[2
]}

Ghimire, Mukti N. ^{[2
]}

Leonard, B. Rogers ^{[2
]}

Siegfried, Blair D. ^{[3
]}

Rangasamy, Murugesan ^{[3
]}

Yang, Yajun ^{[4
]}

Wu, Yidong ^{[4
]}

Gahan, Linda J. ^{[5
]}

Heckel, David G. ^{[6
]}

Bravo, Alejandra ^{[7
]}

Soberon, Mario ^{[7
]}

机构：

[1] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA

[2] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA

[3] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA

[4] Nanjing Agr Univ, Coll Plant Protect, Dept Entomol, Nanjing, Peoples R China

[5] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA

[6] Max Planck Inst Chem Ecol, Dept Entomol, Jena, Germany

[7] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62191, Morelos, Mexico

来源：

NATURE BIOTECHNOLOGY | 2011年 / 29卷 / 12期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

BACILLUS-THURINGIENSIS TOXIN; EUROPEAN CORN-BORER; HELIOTHIS-VIRESCENS LEPIDOPTERA; DIAMONDBACK MOTH LEPIDOPTERA; FIELD-DERIVED STRAIN; OSTRINIA-NUBILALIS LEPIDOPTERA; CROSS-RESISTANCE; SUGARCANE BORER; CRY1AB TOXIN; CADHERIN;

D O I：

10.1038/nbt.1988

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic crops that produce Bacillus thuringiensis (Bt) toxins are grown widely for pest control(1), but insect adaptation can reduce their efficacy(2-6). The genetically modified Bt toxins Cry1AbMod and Cry1AcMod were designed to counter insect resistance to native Bt toxins Cry1Ab and Cry1Ac(7). Previous results suggested that the modified toxins would be effective only if resistance was linked with mutations in genes encoding toxin-binding cadherin proteins(7). Here we report evidence from five major crop pests refuting this hypothesis. Relative to native toxins, the potency of modified toxins was >350-fold higher against resistant strains of Plutella xylostella and Ostrinia nubilalis in which resistance was not linked with cadherin mutations. Conversely, the modified toxins provided little or no advantage against some resistant strains of three other pests with altered cadherin. Independent of the presence of cadherin mutations, the relative potency of the modified toxins was generally higher against the most resistant strains.

引用

页码：1128 / U98

页数：6

共 60 条

[1] Inheritance of resistance to the Cry1Ab Bacillus thuringiensis toxin in Ostrinia nubilalis (Lepidoptera: crambidae) [J].