Characterization of cDNA encoding molt-inhibiting hormone of the crab, Cancer pagurus;: expression of MIH in non-X-organ tissues

被引:30
作者
Lu, WQ
Wainwright, G
Olohan, LA
Webster, SG
Rees, HH
Turner, PC
机构
[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England
[2] Univ Wales, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales
基金
英国生物技术与生命科学研究理事会;
关键词
eyestalk; sinus gland; X-organ; ecdysteroid; molting hormone;
D O I
10.1016/S0378-1119(01)00708-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Synthesis of ecdysteroids (molting hormones) by crustacean Y-organs is regulated by a neuropeptide, molt-inhibiting hormone (MIH), produced in eyestalk neural ganglia. We report here the molecular cloning of a cDNA encoding MIH of the edible crab, Cancer pagurus. Full-length MIH cDNA was obtained by using reverse transcription-polymerise chain reaction (RT-PCR) with degenerate oligonucleotides based upon the amino acid sequence of MIH, in conjunction with 5'- and 3'-RACE. Full-length clones of MIH cDNA were obtained that encoded a 35 amino acid putative signal peptide and the mature 78 amino acid peptide. Of various tissues examined by Northern blot analysis, the X-organ was the sole major site of expression of the MIH gene. However, a nested-PCR approach using non-degenerate MIH-specific primers indicated the presence of MIH transcripts in other tissues. Southern blot analysis indicated a simple gene arrangement with at least two copies of the MIH gene in the genome of C. pagurus. Additional Southern blotting experiments detected MIH-hybridizing bands in another Cancer species, Cancer antennarius and another crab species, Carcinus maenas. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 159
页数:11
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