Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs

被引：26

作者：

Aoyama, C

Nakashima, K

Ishidate, K

机构：

[1] Tokyo Med & Dent Univ, Med Res Inst, Dept Mol Cell Biol, Chiyoda Ku, Tokyo 1010062, Japan

[2] Kyoritsu Coll Pharm, Dept Hyg Chem, Minato Ku, Tokyo 105, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1998年 / 1393卷 / 01期

基金：

日本学术振兴会;

关键词：

choline kinase; ethanolamine kinase; cDNA cloning; isozyme; phosphocholine; phosphoethanolamine; mRNA expression;

D O I：

10.1016/S0005-2760(98)00062-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S, Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 185

页数：7

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