A 96-well assay for uronic acid carbazole reaction

被引：214

作者：

Cesaretti, M ^{[1
]}

Luppi, E ^{[1
]}

Maccari, F ^{[1
]}

Volpi, N ^{[1
]}

机构：

[1] Univ Modena & Reggio Emilia, Dept Biol Anim, I-41100 Modena, Italy

来源：

CARBOHYDRATE POLYMERS | 2003年 / 54卷 / 01期

关键词：

uronic acid; carbazole; glycosaminoglycans; hyaluronic acid; heparin; chondroitin sulfate;

D O I：

10.1016/S0144-8617(03)00144-9

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive and reproducible 96-well assay of uronic acid permitting a rapid processing of a number of samples with a very low consumption of reagents is described-for the determination of complex uronic acid-bearing polyanions such as hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparin. The sensitivity of the reaction was approx. 1 mug for glucuronic acid and 2 mug for complex polysaccharides, with a linear function of glucuronic acid concentration between 1 and 100 mug. The relative coefficient of variations ranged from 1.5 to 8.7% for the assay performed in the 96-well plate. These values were found to be lower than those obtained by the conventional procedure. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：59 / 61

页数：3

共 7 条

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