How an enzyme answers. multiple-choice questions

被引：7

作者：

McCourt, JA ^{[1
]}

Duggleby, RG ^{[1
]}

机构：

[1] Univ Queensland, Sch Mol & Microbial Sci, St Lucia, Qld 4072, Australia

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2005年 / 30卷 / 05期

关键词：

D O I：

10.1016/j.tibs.2005.03.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetohydroxyacid synthase (AHAS) is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids. Interest in the enzyme has escalated over the past 20 years since it was discovered that AHAS is the target of the sulfonylurea and imidazolinone herbicides. However, several questions regarding the reaction mechanism have remained unanswered, particularly the way in which AHAS I chooses' its second substrate. A new method for the detection of reaction intermediates enables calculation of the microscopic rate constants required to explain this phenomenon.

引用

页码：222 / 225

页数：4

共 14 条

[1] PHYSIOLOGICAL IMPLICATIONS OF THE SPECIFICITY OF ACETOHYDROXY ACID SYNTHASE ISOZYMES OF ENTERIC BACTERIA
BARAK, Z
CHIPMAN, DM
GOLLOP, N
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (08) : 3750 - 3756
[2] ACETOHYDROXY ACID SYNTHASE-I, A REQUIRED ENZYME FOR ISOLEUCINE AND VALINE BIOSYNTHESIS IN ESCHERICHIA-COLI K-12 DURING GROWTH ON ACETATE AS THE SOLE CARBON SOURCE
DAILEY, FE
CRONAN, JE
[J]. JOURNAL OF BACTERIOLOGY, 1986, 165 (02) : 453 - 460
[3] Duggleby RG, 2000, J BIOCHEM MOL BIOL, V33, P1
[4] DUGGLEBY RG, 2004, THIAMINE CATALYTIC M, P251
[5] Role of a conserved arginine in the mechanism of acetohydroxyacid synthase - Catalysis of condensation with a specific ketoacid substrate
Engel, S
Vyazmensky, M
Vinogradov, M
Berkovich, D
Bar-Ilan, A
Qimron, U
Rosiansky, Y
Barak, Z
Chipman, DM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (23) : 24803 - 24812
[6] Acetohydroxyacid synthase:: A new enzyme for chiral synthesis of R-phenylacetylcarbinol
Engel, S
Vyazmensky, M
Geresh, S
Barak, Z
Chipman, DM
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 2003, 83 (07) : 833 - 840
[7] Branched-chain amino acid biosynthesis in Salmonella typhimurium:: a quantitative analysis
Epelbaum, S
LaRossa, RA
VanDyk, TK
Elkayam, T
Chipman, DM
Barak, Z
[J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (16) : 4056 - 4067
[8] KINETICS AND MECHANISM OF ACETOHYDROXY ACID SYNTHASE ISOZYME-III FROM ESCHERICHIA-COLI
GOLLOP, N
DAMRI, B
BARAK, Z
CHIPMAN, DM
[J]. BIOCHEMISTRY, 1989, 28 (15) : 6310 - 6317
[9] PHYSIOLOGICAL IMPLICATIONS OF THE SUBSTRATE SPECIFICITIES OF ACETOHYDROXY ACID SYNTHASES FROM VARIED ORGANISMS
GOLLOP, N
DAMRI, B
CHIPMAN, DM
BARAK, Z
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (06) : 3444 - 3449
[10] Homology modeling of the structure of bacterial acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis
Ibdah, M
BarIlan, A
Livnah, O
Schloss, JV
Barak, Z
Chipman, DM
[J]. BIOCHEMISTRY, 1996, 35 (50) : 16282 - 16291

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