Chromatin and RNAi factors protect the C-elegans germline against repetitive sequences

被引：114

作者：

Robert, VJP ^{[1
]}

Sijen, T ^{[1
]}

van Wolfswinkel, J ^{[1
]}

Plasterk, RHA ^{[1
]}

机构：

[1] Hubrecht Lab, NL-3584 CT Utrecht, Netherlands

来源：

GENES & DEVELOPMENT | 2005年 / 19卷 / 07期

关键词：

RNAi; cosuppression; repetitive sequences; transcriptional gene silencing; heterochromatin; C; elegans;

D O I：

10.1101/gad.332305

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Protection of genomes against invasion by repetitive sequences, such as transposons, viruses, and repetitive transgenes, involves strong and selective silencing of these sequences. During silencing of repetitive transgenes, a trans effect ("cosuppression") occurs that results in silencing of cognate endogenous genes. Here we report RNA interference (RNAi) screens performed to catalog genes required for cosuppression in the Caenorhabditis elegans germline. We find factors with a putative role in chromatin remodeling and factors involved in RNAi. Together with molecular data also presented in this study, these results suggest that in C. elegans repetitive sequences trigger transcriptional gene silencing using RNAi and chromatin factors.

引用

页码：782 / 787

页数：6

共 40 条

[1] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].