A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater

被引：30

作者：

Morohoshi, T

Yamashita, T

Kato, J

Ikeda, T

Takiguchi, N

Ohtake, H

Kuroda, A ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Higashihiroshima, Hiroshima 7398530, Japan

[2] Japan Sci & Technol Corp, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2003年 / 95卷 / 06期

关键词：

Pseudomonas putida; Acinetobacter; polyphosphate; phosphate removal; activated sludge; pho regulon;

D O I：

10.1016/S1389-1723(03)80177-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The biological process for phosphorus removal from wastewater is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We previously showed that a phoU mutation leads to polyP accumulation in Escherichia coli. The phoU mutant could be easily screened on agar plates containing 5-bromo-4-chloro-3-indolyl-phosphate (X-P-i) after N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. Here, we demonstrate that this method is also useful for screening polyP-accumulating mutants of bacterial strains isolated from soil and activated sludge samples.

引用

页码：637 / 640

页数：4

共 13 条

[1]

A potential phosphate crisis [J].