Transformation of the phytopathogen Mycosphaerella graminicola to carbendazim and hygromycin B resistance

被引：16

作者：

Payne, AC ^{[1
]}

Grosjean-Cournoyer, MC

Hollomon, DW

机构：

[1] Univ Bristol, Dept Agr Sci, Long Ashton Res Stn, IACR, Bristol BS41 9AF, Avon, England

[2] Rhone Poulenc AGRo, F-69263 Lyon 09, France

来源：

CURRENT GENETICS | 1998年 / 34卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Septoria tritici; beta-tubulin;

D O I：

10.1007/s002940050372

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The fungal wheat pathogen Mycosphaerella graminicola was transformed to carbendazim and hygromycin B resistance. A beta-tubulin gene from a M. graminicola strain resistant to the fungicide carbendazim was cloned and used to transform a sensitive strain to carbendazim resistance. Hygromycin B-resistant transformants (up to 8 per mu g Of transforming DNA) arose at a higher rate than beta-tubulin transformants (up to 0.6 per mu g of DNA). Transformants were able to infect wheat and were morphologically similar to recipient strains.

引用

页码：100 / 104

页数：5

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