Microtubular organization in tobacco cells: Heat-shock protein 90 can bind to tubulin in vitro

被引:11
作者
Freudenreich, A [1 ]
Nick, P [1 ]
机构
[1] Inst Biol 2, D-79104 Freiburg, Germany
来源
BOTANICA ACTA | 1998年 / 111卷 / 04期
关键词
chaperones; heat-shock protein 90 (HSP90); microtubules; tobacco cells;
D O I
10.1111/j.1438-8677.1998.tb00708.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The heat-shock protein 90 (HSP90) from tobacco VBI-O cells specifically binds to nitrocellulose that had been coated with polymerized microtubules or tubulin dimers. HSP90 is expressed preferentially during cell division and becomes downregulated during cell elongation. HSP90 cofractionates with tubulin dimers during affinity chromatography with sepharose coupled to the tubulin-binding drug ethyl N-phenylcarbamate (EPC). Binding of HSP90 to EPC-sepharose depends on the presence of tubulin. Antibodies against tubulin and HSP90 immunoadsorb HSP90 and tubulin, respectively. These results demonstrate that HSP90 behaves as a microtubule-binding protein in vitro.
引用
收藏
页码:273 / 279
页数:7
相关论文
共 23 条
[1]  
ANSORGE W, 1983, ELECTROPHORESIS, V82, P235
[2]   ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].
BENSADOUN, A ;
WEINSTEIN, D .
ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :241-250
[3]   DIRECT STOICHIOMETRIC EVIDENCE THAT THE UNTRANSFORMED MR 300 000, 9S, GLUCOCORTICOID RECEPTOR IS A CORE UNIT DERIVED FROM A LARGER HETEROMERIC COMPLEX [J].
BRESNICK, EH ;
DALMAN, FC ;
PRATT, WB .
BIOCHEMISTRY, 1990, 29 (02) :520-527
[4]  
Czar MJ, 1996, EUR J CELL BIOL, V70, P322
[5]  
GEORGOPOULOS C, 1993, ANNU REV CELL BIOL, V9, P601, DOI 10.1146/annurev.cellbio.9.1.601
[6]  
GODDARD RH, 1994, PLANT PHYSIOL, V104, P1
[7]   Developmental and light-dependent changes of the cytosolic chaperonin containing TCP-1 (CCT) subunits in maize seedlings, and the localization in coleoptiles [J].
Himmelspach, R ;
Nick, P ;
Schäfer, E ;
Ehmann, B .
PLANT JOURNAL, 1997, 12 (06) :1299-1310
[8]   ROLE OF THE PROTEIN CHAPERONE YDJ1 IN ESTABLISHING HSP90-MEDIATED SIGNAL-TRANSDUCTION PATHWAYS [J].
KIMURA, Y ;
YAHARA, I ;
LINDQUIST, S .
SCIENCE, 1995, 268 (5215) :1362-1365
[9]   2 MAMMALIAN HEAT-SHOCK PROTEINS, HSP90 AND HSP100, ARE ACTIN-BINDING PROTEINS [J].
KOYASU, S ;
NISHIDA, E ;
KADOWAKI, T ;
MATSUZAKI, F ;
IIDA, K ;
HARADA, F ;
KASUGA, M ;
SAKAI, H ;
YAHARA, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8054-8058
[10]   COLD-INDUCED ACCUMULATION OF HSP90 TRANSCRIPTS IN BRASSICA-NAPUS [J].
KRISHNA, P ;
SACCO, M ;
CHERUTTI, JF ;
HILL, S .
PLANT PHYSIOLOGY, 1995, 107 (03) :915-923