Chromosome landing at the barley Rar1 locus

被引：28

作者：

Lahaye, T ^{[1
]}

Shirasu, K ^{[1
]}

Schulze-Lefert, P ^{[1
]}

机构：

[1] John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

MOLECULAR AND GENERAL GENETICS | 1998年 / 260卷 / 01期

关键词：

resistance reaction; powdery mildew; yeast artificial chromosome (YAC); map-based cloning; cleaved amplified polymorphic sequence (CAPS);

D O I：

10.1007/s004380050874

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.

引用

页码：92 / 101

页数：10

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