Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails -: art. no. R17

被引：62

作者：

Kunitomo, H

Uesugi, H

Kohara, Y

Iino, Y

机构：

[1] Univ Tokyo, Mol Genet Res Lab, Bunkyo Ku, Tokyo 1130033, Japan

[2] Natl Inst Genet, Genome Biol Lab, Mishima, Shizuoka 4118540, Japan

来源：

GENOME BIOLOGY | 2005年 / 6卷 / 02期

关键词：

D O I：

10.1186/gb-2005-6-2-r17

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly( A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.

引用

页数：13

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