Quantitative analysis of anti-emetic principle in the tubers of Pinellia ternata by enzyme immunoassay

被引：39

作者：

Kurata, K

Tai, T

Yang, Y

Kinoshita, K

Koyama, K

Takahashi, K

Watanabe, K

Nunoura, Y

机构：

[1] Kotaro Pharmaceut Co Ltd, Cent Res Lab, Takatsuki, Osaka 5690022, Japan

[2] Meiji Coll Pharm, Dept Pharmacognosy & Phytochem, Tokyo 154, Japan

[3] Chiba Univ, Fac Pharmaceut Sci, Chiba 260, Japan

来源：

PLANTA MEDICA | 1998年 / 64卷 / 07期

关键词：

anti-emetic; quantitative analysis; enzyme immunoassay; Pinellia ternata (Thunb.) Breit; Araceae;

D O I：

10.1055/s-2006-957539

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A crude globulin fraction was isolated from the tubers of Pinellia ternata (Thunb.) Breit. Tubers of P. ternata are one of the well known Chinese traditional medicines, It is used on account of its anti-emetic effect. 6KDP was separated by means of gel filtration and chromatography on CM-Toyopearl 650 M from the crude globulin fraction. 6KDP is a glycoprotein of M.W. 6 kDa. 6KDP has an anti-emetic activity and hemagglutination activity. Both activities dropped during heat treatment. For the purpose to evaluate the quality of the powdered tubers of P. ternata, the quantitative analysis by means of FIA was established. The contents of 6KDP varied from 5.75 to 8.30 %. The assay demonstrated a low cross-reaction with the crude globulin fraction of Colocasia antiquorum, a member of the same family as P. ternata. 6KDP is one of major proteins in the tubers of P. ternata. Thus 6KDP is a marker for quantitative analysis of the tubers of P. ternata.

引用

页码：645 / 648

页数：4

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