The TatBC complex formation suppresses a modular TatB-multimerization in Escherichia coli

被引：28

作者：

Behrendt, Jana ^{[1
]}

Lindenstrauss, Ute ^{[1
]}

Brueser, Thomas ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Biol Microbiol, D-06120 Halle, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 21期

关键词：

protein transport; translocon assembly; membrane protein complexes; twin-arginine translocation;

D O I：

10.1016/j.febslet.2007.07.045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Twin-arginine translocation (Tat) systems allow the translocation of folded proteins across biological membranes of most prokaryotes. In proteobacteria, a TatBC complex binds Tat substrates and initiates their translocation after recruitment of the component TatA. TatA and TatB belong to one protein family, but only TatB forms stable complexes with TatC. Here we show that TatB builds up TatA-like modular complexes in the absence of TatC. This TatB ladder ranges from about 100 to over 880 kDa with 105 +/- 10 kDa increments. TatC alone can form a 250 kDa complex which could be a scaffold that can recruit TatB to form defined TatBC complexes. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4085 / 4090

页数：6

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