Upscaling and automation of electrophysiology: Toward high throughput screening in ion channel drug discovery

被引:52
作者
Asmild, M
Oswald, N
Krzywkowski, KM
Friis, S
Jacobsen, RB
Reuter, D
Taboryski, R
Kutchinsky, J
Vestergaard, RK
Schroder, RL
Sorensen, CB
Bech, M
Korsgaard, MPG
Willumsen, NJ
机构
[1] Sophion Biosci, DK-2750 Ballerup, Denmark
[2] NeuroSearch, Ballerup, Denmark
[3] Univ Copenhagen, August Krogh Inst, DK-1168 Copenhagen, Denmark
关键词
drug discovery; HTS electrophysiology; ion channels; patch clamp; silicon chip;
D O I
10.1080/10606820308258
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1(TM), and a silicon chip-based system QPatch(TM). The degree of automation spans from semi-automation (Apatchi-1(TM)) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96(TM)) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs.
引用
收藏
页码:49 / 58
页数:10
相关论文
共 11 条
  • [1] Stable expression of the human large-conductance Ca2+-activated K+ channel alpha- and beta-subunits in HEK293 cells
    Ahring, PK
    Strobaek, D
    Christophersen, P
    Olesen, SP
    Johansen, TE
    [J]. FEBS LETTERS, 1997, 415 (01): : 67 - 70
  • [2] Whole cell patch clamp recording performed on a planar glass chip
    Fertig, N
    Blick, RH
    Behrends, JC
    [J]. BIOPHYSICAL JOURNAL, 2002, 82 (06) : 3056 - 3062
  • [3] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES
    HAMILL, OP
    MARTY, A
    NEHER, E
    SAKMANN, B
    SIGWORTH, FJ
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02): : 85 - 100
  • [4] JENSEN BS, 1998, AM J PHYSIOL, V257, pC848
  • [5] Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells
    Klemic, KG
    Klemic, JF
    Reed, MA
    Sigworth, FJ
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (6-7) : 597 - 604
  • [6] OWEN D, 2002, DRUG DISCOVERY W SPR
  • [7] Schmidt C, 2000, ANGEW CHEM INT EDIT, V39, P3137, DOI 10.1002/1521-3773(20000901)39:17<3137::AID-ANIE3137>3.0.CO
  • [8] 2-D
  • [9] Sogaard R, 2001, AM J PHYSIOL-CELL PH, V280, pC859
  • [10] WILLUMSEN NJ, 2003, RECEPTORS CHANNELS