Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels

被引：269

作者：

Au, Patrick ^{[1
,2
]}

Daheron, Laurence M. ^{[3
,4
]}

Duda, Dan G. ^{[1
]}

Cohen, Kenneth S. ^{[3
,4
]}

Tyrrell, James A. ^{[1
]}

Lanning, Ryan M. ^{[1
,2
]}

Fukumura, Dai ^{[1
]}

Scadden, David T. ^{[3
,4
]}

Jain, Rakesh K. ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiat Oncol,Edwin L Steel Lab, Boston, MA 02114 USA

[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] Harvard Univ, Massachusetts Gen Hosp, Ctr Regenerat Med, Sch Med, Cambridge, MA 02138 USA

[4] Harvard Stem Cell Inst, Cambridge, MA USA

来源：

BLOOD | 2008年 / 111卷 / 03期

关键词：

D O I：

10.1182/blood-2007-06-094318

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Tissue engineering requires formation of a de novo stable vascular network. Because of their ability to proliferate, differentiate into endothelial cells, and form new vessels, blood-derived endothelial progenitor cells (EPCs) are attractive source of cells for use in engineering blood vessels. However, the durability and function of EPC-derived vessels implanted in vivo are unclear. To this end, we directly compared formation and functions of tissue-engineered blood vessels generated by peripheral blood- and umbilical cord blood-derived EPCs in a model of in vivo vasculogenesis. We found that adult peripheral blood EPCs form blood vessels that are unstable and regress within 3 weeks. In contrast, umbilical cord blood EPCs form normal-functioning blood vessels that last for more than 4 months. These vessels exhibit normal blood flow, perm-selectivity to macromolecules, and induction of leukocyte-endothelial interactions in response to cytokine activation similar to normal vessels. Thus, umbilical cord blood EPCs hold great therapeutic potential, and their use should be pursued for vascular engineering.

引用

页码：1302 / 1305

页数：4

共 14 条

[1]

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