Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes

被引：125

作者：

Härtl, B ^{[1
]}

Wehrl, W ^{[1
]}

Wiegert, T ^{[1
]}

Homuth, G ^{[1
]}

Schumann, W ^{[1
]}

机构：

[1] Univ Bayreuth, Inst Genet, D-95440 Bayreuth, Germany

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 08期

关键词：

D O I：

10.1128/JB.183.8.2696-2699.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

The Bacillus subtilis lacA gene, coding for beta -galactosidase, has been explored as a new site able to accept DNA sequences from nonreplicating delivery vectors. Two such delivery expression vectors have been constructed and shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a regulatory gene at the lacA locus was able to control the expression of a transcriptional fusion at the amyE locus. These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes.

引用

页码：2696 / 2699

页数：4

共 13 条

[1]

Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR [J].