Adsorption behavior of urokinase by polypropylene film modified with amino acids as affinity groups

In order to prepare a new-type adsorbent with an affinity ligand, polypropylene films modified with amino acid groups such as L-phenylalanine (Phe), D,L-Phe, L-cysteine (Cys), and D,L-tryptophane (Try), were prepared by radiation-induced grafting of glycidyl methacrylate (GMA) onto polypropylene (PP) films and subsequent amination of poly-GMA graft chains. The physical and chemical properties of the GMA-grafted PP film and the PP him modified with amino acid groups were investigated by IR and XPS. The adsorption of urokinase for the PP films modified with four kinds of amino acid groups were examined under various conditions, such as the contents of the amino acid group and pH value. The adsorption of urokinase increased with the increasing content of the amino acid group. The adsorption of the PP film modified with four kinds of amino acid groups was in the following order: L-Phe > D,L-Phe > D,L-Try > L-Cys. The adsorption amounts of urokinase by the PP film modified with four kinds of amino acid groups at pH 7.3 was higher than that at pH 9.0. (C) 2001 Elsevier Science B.V. All rights reserved.