Expression of insulin-like growth factor I by cultured skin substitutes does not replace the physiologic requirement for insulin in vitro

Clinical efficacy of cultured skin substitutes may be increased if their carbohydrate metabolism is optimized by understanding whether endogenous insulin-like growth factor I can substitute for exogenous insulin. Cultured skin substitutes were prepared and incubated at the air-liquid interface for 4 wk in media containing 0.5 or 5 mug per mi insulin, 10 or 50 ng per mi insulin-like growth factor I, or 0 insulin and 0 insulin-like growth factor I (negative control). In situ hybridization showed that the epidermal and dermal cultured skin substitute components express insulin-like growth factor I mRNA throughout the 28 d interval. Immunohistochemistry confirmed the expression of insulin-like growth factor I protein by the human keratinocytes and fibroblasts in cultured skin substitutes. Insulin-Like growth factor I at 10 or 30 ng per mi could partially replace insulin in a clonal assay of keratinocyte growth. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays showed significantly higher values in cultured skin substitutes incubated with insulin at incubation days 14 and 28 compared to negative control or the 10 ng per mi insulin-like growth factor I condition. Cultured skin substitutes incubated in 50 ng per mi insulin-like growth factor I had MTT values similar to the insulin-treated cultured skin substitutes at day 14, but were significantly lower by day 28, Light microscopy agreed with MTT data showing that cultured skin substitutes grown with insulin media had multiple layers of nucleated keratinocytes and stratum corneum at days 14 and 28, The negative control and 10 ng per mi insulin-like growth factor I exhibited poor cultured skin substitute epidermal morphology throughout the experiment, In contrast, the cultured skin substitutes in 50 ng per mi insulin-like growth factor I were similar to the insulin-treated cultured skin substitutes at day 14, but by day 28 had deteriorated to resemble the negative control. Bromodeoxyuridine incorporation at day 28 was significantly higher for 5 mug per mi insulin cultured skin substitutes versus all other treatment groups, These data suggest. that medium. containing 5 mug per mi insulin supports greater physiologic stability in cultured skin substitutes over time, and that expression of insulin-like growth factor I by keratinocytes and fibroblasts in cultured skin substitutes is not sufficient to fully replace the requirement for exogenous insulin in vitro.