Changes in nitric oxide synthesis and epileptic activity in the contralateral hippocampus of rats following intrahippocampal kainate injection

被引:51
作者
Yasuda, H [1 ]
Fujii, M [1 ]
Fujisawa, H [1 ]
Ito, H [1 ]
Suzuki, M [1 ]
机构
[1] Yamaguchi Univ, Sch Med, Dept Neurosurg, Yamaguchi 7558505, Japan
关键词
hippocampus; kainate; microdialysis; nitric oxide; seizure;
D O I
10.1046/j.1528-1157.2001.083032.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Purpose: To investigate the effects of nitric oxide (NO) on seizure activity observed in brain areas that are remote from a primary epileptic focus. Methods: Following an injection of kainate (concentration 1 mg/ml, volume 1 mul) in the rat hippocampus, we measured NO synthesis in the contralateral hippocampus and epileptic activity by electroencephalogram (EEG). The NO end products, nitrite and nitrate, were measured by in vivo microdialysis combined with an automated NO end-product analyzer and then used as indices of NO synthesis. We also assessed the effect of a specific inhibitor of neuronal NO synthase (NOS) on both the epileptic activity and NO synthesis in the contralateral hippocampus. For this assessment, we administered 7-nitroindazole (7-NI) (50 mg/kg) intraperitoneally 30 min before the kainate injection. Results: Epileptic discharges in the contralateral hippocampus were frequently observed 90 min after unilateral hippocampus kainate injection. The duration of these discharges gradually increased until 240 min after the kainate injection. The NO end-product levels increased immediately after kainate injection and continued to increase gradually throughout the experiments, to a maximum of 213% of the base level. This elevation of NO end products was followed by epileptic discharges. Both the seizure activity and the elevation of contralateral hippocampus NO end-product levels were markedly attenuated in the animals that received 7-NI. Conclusions: The results suggest that remote seizure activity caused by the transneuronal spread of kainate-induced discharges may be related to NO derived from neuronal NOS.
引用
收藏
页码:13 / 20
页数:8
相关论文
共 52 条
[1]  
Amaral David G., 1995, P443
[2]   TACRINE-INDUCED SEIZURES AND BRAIN-DAMAGE IN LICL-TREATED RATS CAN BE PREVENTED BY N-OMEGA-NITRO-L-ARGININE METHYL-ESTER [J].
BAGETTA, G ;
IANNONE, M ;
SCORSA, AM ;
NISTICO, G .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1992, 213 (02) :301-304
[3]   APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].
BECKMAN, JS ;
BECKMAN, TW ;
CHEN, J ;
MARSHALL, PA ;
FREEMAN, BA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1620-1624
[4]   THE ROLE OF EPILEPTIC ACTIVITY IN HIPPOCAMPAL AND REMOTE CEREBRAL-LESIONS INDUCED BY KAINIC ACID [J].
BENARI, Y ;
TREMBLAY, E ;
OTTERSEN, OP ;
MELDRUM, BS .
BRAIN RESEARCH, 1980, 191 (01) :79-97
[5]   A SYNAPTIC MODEL OF MEMORY - LONG-TERM POTENTIATION IN THE HIPPOCAMPUS [J].
BLISS, TVP ;
COLLINGRIDGE, GL .
NATURE, 1993, 361 (6407) :31-39
[6]   NITRIC-OXIDE - AN ENDOGENOUS ANTICONVULSANT SUBSTANCE [J].
BUISSON, A ;
LAKHMECHE, N ;
VERRECCHIA, C ;
PLOTKINE, M ;
BOULU, RG .
NEUROREPORT, 1993, 4 (04) :444-446
[7]   ATROPHY OF MESIAL STRUCTURES IN PATIENTS WITH TEMPORAL-LOBE EPILEPSY - CAUSE OR CONSEQUENCE OF REPEATED SEIZURES [J].
CENDES, F ;
ANDERMANN, F ;
GLOOR, P ;
LOPESCENDES, I ;
ANDERMANN, E ;
MELANSON, D ;
JONESGOTMAN, M ;
ROBITAILLE, Y ;
EVANS, A ;
PETERS, T .
ANNALS OF NEUROLOGY, 1993, 34 (06) :795-801
[8]   Regulation of glutamate release by presynaptic kainate receptors in the hippocampus [J].
Chittajallu, R ;
Vignes, M ;
Dev, KK ;
Barnes, JM ;
Collingridge, GL ;
Henley, JM .
NATURE, 1996, 379 (6560) :78-81
[9]   NITRIC-OXIDE - FOE OR FRIEND TO THE INJURED BRAIN [J].
CHOI, DW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :9741-9743
[10]   THE ROLE OF THE REACTIONS OF (NO)-N-CENTER-DOT WITH SUPEROXIDE AND OXYGEN IN BIOLOGICAL, SYSTEMS - A KINETIC APPROACH [J].
CZAPSKI, G ;
GOLDSTEIN, S .
FREE RADICAL BIOLOGY AND MEDICINE, 1995, 19 (06) :785-794