The N54 mutant of Gαs has a conditional dominant negative phenotype which suppresses hormone-stimulated but not basal cAMP levels

被引：18

作者：

Cleator, JH ^{[1
]}

Mehta, ND ^{[1
]}

Kurtz, DT ^{[1
]}

Hildebrandt, JD ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Cell & Mol Pharmacol, Charleston, SC 29425 USA

来源：

FEBS LETTERS | 1999年 / 443卷 / 02期

关键词：

G protein; hormone-stimulated cAMP level;

D O I：

10.1016/S0014-5793(98)01704-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The phenotype of a Ser to Asn mutation at position 54 of the alpha subunit of G(s) (N54-alpha(s)) was characterized in transient transfection experiments in COS and HEK293 cells, Expression of either wild type or N54-alpha(s) increased basal cAMP levels. In contrast, expression of wild type alpha(s) potentiated agonist-stimulated cAMP levels, while expression of N54-alpha(s) caused a decrease. Thus, the N54-alpha(s) mutant possesses a conditional dominant negative phenotype, suppressing preferentially hormone-stimulated effects. (C) 1999 Federation of European Biochemical Societies.

引用

页码：205 / 208

页数：4

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