Hard tissue regeneration capacity of apical pulp derived cells (APDCs) from human tooth with immature apex

被引：97

作者：

Abe, Shigehiro ^{[1
]}

Yamaguchi, Satoshi ^{[1
]}

Watanabe, Akihiko ^{[2
]}

Hamada, Keiichi ^{[1
]}

Amagasa, Teruo ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch, Div Maxillofacial & Neck Reconstruct, Bunkyo Ku, Tokyo 1138549, Japan

[2] Tokyo Med & Dent Univ, Inst Med & Dent Engn, Div Organ Mat, Tokyo 1010062, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 01期

关键词：

dental pulp; bond; dentin; hydroxyapatite; tissue engineering;

D O I：

10.1016/j.bbrc.2008.04.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Recent studies indicate that dental pulp is a new source of adult stem cells. The human tooth with an immature apex is a developing organ, and the apical pulp of this tooth may contain a variety of progenitor/stem cells, which participate in root formation. We investigated the hard tissue regeneration potential of apical pulp derived cells (APDCs) from human tooth with an immature apex. APDCs cultured with a mineralization-promoting medium showed alkaline phosphatase activity in porous hydroxyapatite (HA) scaffolds. The composites of APDCs and HA were implanted subcutaneously in immunocompromised rats and harvested at 12 weeks after implantation. In histological analysis, the APDCs/HA composites exhibited bone- and dentine-like mineralized tissues in the pore areas of HA. This study suggests that the human tooth with an immature apex is an effective source of cells for hard tissue regeneration. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：90 / 93

页数：4

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