High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivation-independent approach

被引：102

作者：

Gao, XX ^{[1
]}

Zhou, H ^{[1
]}

Xu, DY ^{[1
]}

Yu, CH ^{[1
]}

Chen, YQ ^{[1
]}

Qu, LH ^{[1
]}

机构：

[1] Zhongshan Univ, State Key Lab Biocontrol, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 249卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Heterosmilax japonica Kunth; endophytic fungi; rDNA ITS; phylogenetic analysis;

D O I：

10.1016/j.femsle.2005.06.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Heterosmilax japonica Kunth is well recognized for its diuretic effects in China. However, little is known about its endophytic fungi. In this study, microbial communities inhabiting the stems of H. japonica in spring and summer were investigated by light microscopy and cultivation-independent approaches, such as RFLP analysis and sequencing of rDNA ITS library. Molecular phylogenetic analysis showed that a broad spectrum of fungi, including Mycosphaerella, Phomopsis, Aureobasidium, Cladosporium, Glomerella, Botryosphaeria, Guignardia, is able to colonize the plants internally. Particularly, several rDNA sequences determined in this study like YJ4-61 are not specifically affiliated with any currently documented fungal sequences in the public database. Several sequence types, such as YJ4-9 and YJ4-70, are significantly similar to some uncultured environmental samples. Furthermore, our result also showed that the samples collected in spring harbored more abundant endophytic populations than that in summer, implying a seasonal fluctuation for the endophytes in H. japonica. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：255 / 266

页数：12

共 47 条

[1]

MORPHOGENESIS AND ADHESION OF AUREOBASIDIUM-PULLULANS [J].