Asymmetric protoplast fusion between sweet potato and its relatives, and plant regeneration

被引：11

作者：

Belarmino, MM

Abe, T

Sasahara, T

机构：

[1] YAMAGATA UNIV,FAC AGR,LAB PLANT BREEDING,TSURUOKA 997,JAPAN

[2] VISCA,PHILIPPINE ROOT CROP RES & TRAINING CTR,LEYTE 6521A,PHILIPPINES

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1996年 / 46卷 / 03期

关键词：

electrofusion; iodoacetamide; Ipomoea; sweet potato; polyethylene glycol; peroxidase; X-ray;

D O I：

10.1007/BF02307095

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Experiments were conducted to asymmetrically fuse protoplasts from sweet potato (Ipomoea batatas L. Lam.) and its wild relatives I. trifida Don. and I. lacunosa L. Protoplasts of sweet potato were treated with iodoacetamide, whereas those of I. trifida Don. and I. lacunosa L. were irradiated with X-rays. The asymmetric protoplast fusion was carried out by the electrofusion method and by polyethylene glycol treatment. Electrically-fused protoplasts initiated cell division, and then formed calli earlier than the polyethylene glycol-fused protoplasts. Plant regeneration occurred only in electrofused calli, suggesting that polyethylene glycol had some toxic effect on plant regeneration ability. Analysis of peroxidase isozymes confirmed the interspecific hybrid characteristics of both the fusion-derived calli and regenerated plants.

引用

页码：195 / 202

页数：8

共 25 条

[1]

BELARMINO MM, 1992, JPN J BREED, V42, P109

[2]

TUNICAMYCIN-INHIBITED CARROT SOMATIC EMBRYOGENESIS CAN BE RESTORED BY SECRETED CATIONIC PEROXIDASE ISOENZYMES [J].