Anti-inflammatory activity of Angelica dahurica ethanolic extract on RAW264.7 cells via upregulation of heme oxygenase-1

被引：88

作者：

Lee, Mee-Young ^{[1
]}

Lee, Jin-Ah ^{[1
]}

Seo, Chang-Seob ^{[1
]}

Ha, Heykyung ^{[1
]}

Lee, Hoyoung ^{[1
]}

Son, Jong-Keun ^{[2
]}

Shin, Hyeun-Kyoo ^{[1
]}

机构：

[1] Korea Inst Oriental Med, Herbal Med EBM Res Ctr, Taejon 305811, South Korea

[2] Yeungnam Univ, Coll Pharm, Gyongsan 712749, Gyeongbuk, South Korea

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2011年 / 49卷 / 05期

关键词：

Angelica dahurica; iNOS; COX-2; HO-1; IL4;

D O I：

10.1016/j.fct.2011.01.010

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

In this study, we analyzed the anti-inflammatory effects of Angelica dahurica Bentham et Hooker ethanolic extract (ADEE) on RAW264.7 cells, to understand the mechanism underlying its observed effects. ADEE inhibited cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to the suppression of COX-2-derived prostaglandin E-2 and iNOS-derived production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. These inhibitory effects of ADEE were accompanied by the reduced production of tumor necrosis factor a and interleukin (IL)-6. ADEE also inhibited nuclear factor kappa B (NF kappa B) translocation to the nucleus by interrupting inhibitor kappa B alpha (I kappa B alpha) degradation. ADEE upregulated heme oxygenase 1 expression, and treatment with tin protoporphyrin IX (SnPP), a selective inhibitor of HO-1, reversed the LPS-induced generation of proinflammatory cytokines. ADEE also induced IL-4 and IL-5 expression in concanavalin-A-stimulated splenocytes. These results suggest that ADEE has anti-inflammatory activity, which acts via the suppression of the NF-kappa B pathway. (C) 2011 Published by Elsevier Ltd.

引用

页码：1047 / 1055

页数：9