Polyvinyl-alcohol-based magnetic beads for rapid and efficient separation of specific or unspecific nucleic acid sequences

被引：76

作者：

Oster, J ^{[1
]}

Parker, J ^{[1
]}

Brassard, LA ^{[1
]}

机构：

[1] Chemagen Biopolymer Technol AG, D-52499 Baesweiler, Germany

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2001年 / 225卷 / 1-2期

关键词：

magnetic beads; polyvinyl alcohol (PVA); PVA microspheres; nucleic acid purification; DNA separation; bacteria detection; automation;

D O I：

10.1016/S0304-8853(00)01243-9

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The versatile application of polyvinyl-alcohol-based magnetic M-PVA beads is demonstrated in the separation of genomic DNA, sequence specific nucleic acid purification, and binding of bacteria for subsequent DNA extraction and detection. It is shown that nucleic acids can be obtained in high yield and purity using M-PVA beads, making sample preparation efficient, fast and highly adaptable for automation processes. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：145 / 150

页数：6

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