DEAE-Dextran enhanced firefly bioluminescent assay of ATP

被引:10
作者
Kamidate, T
Kinkou, T
Watanabe, H
机构
[1] Faculty of Engineering, Hokkaido University
关键词
D O I
10.1246/cl.1996.237
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Diethylaminoethyl (DEAE) - Dextran (Dx) enhanced the intensity of bioluminescence from firefly luciferase-luciferin reaction with adenosine-5'-triphosphate (ATP). The detection limit for ATP was 5 x 10(-12) M in the presence of DEAE-Dx, and was improved a factor of three times compared to that in the absence of DEAE-Dx.
引用
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页码:237 / 238
页数:2
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