Effects of genistein on expression cell of bone markers during MC3T3-E1 osteoblastic differentiation

被引:59
作者
Chen, XW
Garner, SC
Quarles, LD
Anderson, JJB [1 ]
机构
[1] Univ N Carolina, Sch Publ Hlth, Dept Nutr, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Sch Med, Dept Nutr, Chapel Hill, NC 27599 USA
[3] Technol Planning & Management Corp, Durham, NC 27703 USA
[4] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA
关键词
genistein; estrogen receptor-alpha and beta; osteoblast; interleukin-6; osteoprotegerin;
D O I
10.1016/S0955-2863(03)00056-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this in vitro study, the hypothesis that the beneficial effects of dietary genistein on bone are through the modulation of the bone marker synthesis by ostcoblastic MC3T3-E1 cells was tested, and the possible roles of estrogen receptors in the actions of genistein on osteoblastic cells were also examined. Interleukin-6 production was decreased 40% to 60% in osteoblastic cells treated with genistein from either day 8-16 or day 12-16, at dietarily achievable concentrations (10(-10) to 10(-8) M) (P<0.05). The mRNA expression of osteoprotegerin increased about 140% in cells treated from with genistein day 4-8 at a concentration of 10(-8) M (P<0.05). The ratio of estrogen receptor-a to 0 expression increased 10-fold from day 0 to 12 of culture (P<0.05). Correlating with this time-dependent variation in estrogen receptor expression, treatments of 17beta-estradiol and genistein had opposite dose patterns on the ratio of estrogen receptor-a to 0 expression following treatment from day 4 to 6 compared to from day 0 to 2. The addition of ICI-182,780, an estrogen receptor blocker, reduced the inhibitory effect of genistein on IL-6 production by 30-50%. In summary, these findings suggest that the beneficial skeletal effects of genistein, at dietarily achievable levels, appear to be mediated, at least in part, by interleukin-6 and osteoprotegerin, and estrogen receptors play important roles in the inhibition of interleukin-6 synthesis by genistein in osteoblastic MC3T3-E1 cells. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:342 / 349
页数:8
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