Angelica sinensis modulates migration and proliferation of gastric epithelial cells

被引：58

作者：

Ye, YN

Koo, MWL

Li, Y

Matsui, H

Cho, CH

机构：

[1] Zhejiang Univ, Dept Pharmacol, Hangzhou 310027, Peoples R China

[2] Univ Tsukuba, Inst Clin Med, Tsukuba Science City, Ibaraki 3050006, Japan

来源：

LIFE SCIENCES | 2001年 / 68卷 / 08期

关键词：

Angelica sinensis; gastric epithelial cells; migration; proliferation;

D O I：

10.1016/S0024-3205(00)00994-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A crude extract from Angelica sinensis (ASCE), which mainly consists of polysaccharides, pre vents ethanol- or indomethacin-induced gastric mucosal damage and promotes ulcer healing. The aim of this study was to test the hypothesis that ASCE has a direct stimulating effect on gastric epithelial cells for wound healing. We found that ASCE significantly promoted the migration of epithelial cells over an artificial wound on the surface of an RGM-1 monolayer. The extract also stimulated DNA synthesis in a dose-dependent manner and concomitantly increased EGF mRNA expression. Go-incubation of ASCE with anti-EGF antibody reduced the speed of mi,oration and the DNA synthesis, which however were still higher than the control without ASGE. These results strongly suggest that ASCE has a direct wound healing effect on gastric mucosa, and this is acting partially through an EGF-mediated pathway. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：961 / 968

页数：8

共 17 条

[1] CELL-MIGRATION IS ESSENTIAL FOR SUSTAINED GROWTH OF KERATINOCYTE COLONIES - THE ROLES OF TRANSFORMING GROWTH FACTOR-ALPHA AND EPIDERMAL GROWTH-FACTOR [J].