Arginine decarboxylase transgene expression and analysis of environmental stress tolerance in transgenic rice

被引:139
作者
Roy, M
Wu, R
机构
[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
[2] Bose Inst, Dept Bot, Kolkata 700009, W Bengal, India
关键词
arginine decarboxylase; polyamine; transgenic rice; stress tolerance;
D O I
10.1016/S0168-9452(01)00337-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arginine decarboxylase (ADC) cDNA from oat (Avena sativa L.) was introduced into rice (Oryza sativa L.) genome by an Agrobacterium-mediated transformation method. Expression of the ADC transgene under the control of an ABA-inducible promoter led to stress-induced upregulation of ADC activity and polyamine accumulation in transgenic rice plants. Second-generation (R1) transgenic rice plants showed an increase in biomass under salinity-stress conditions, as compared to the non-transformed control plants. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:869 / 875
页数:7
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