Immuno-enhancing activity of sulfated Auricularia auricula polysaccharides

被引：160

作者：

The Luong Nguyen ^{[1
]}

Wang, Deyun ^{[1
]}

Hu, Yuanliang ^{[1
]}

Fan, Yunpeng ^{[1
]}

Wang, Junmin ^{[1
]}

Abula, Saifuding ^{[1
]}

Guo, Liwei ^{[1
]}

Zhang, Jing ^{[1
]}

Khakame, Shem Kakai ^{[2
]}

Bao Khanh Dang ^{[3
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

[3] Univ Montreal, Fac Vet Med, Dept Biomed, St Hyacinthe, PQ J2S 7C6, Canada

来源：

CARBOHYDRATE POLYMERS | 2012年 / 89卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Auricularia auricula polysaccharides; Sulfation modification; Lymphocyte proliferation; Antibody titer; LYMPHOCYTE-PROLIFERATION; ANTIOXIDANT ACTIVITIES; IN-VITRO; CHICKEN; GLUCAN; JUDAE; VACCINATION;

D O I：

10.1016/j.carbpol.2012.03.082

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The crude total Auricularia auricula polysaccharide (AAP(ct)) was extracted by water decoction and ethanol precipitation, protein was removed to obtain total A. auricula polysaccharide (AAP(t)), then was graded into AAP(1) and AAP(2) through column chromatography. sAAP(t), sAAP(1) and sAAP(2) were prepared by chlorosulfonic acid-pyridine method. In vitro test, the effects of sAAP(t), sAAP(t), sAAP(2), AAP(t), AAP(1) and AAP(2), on chicken peripheral lymphocytes proliferation were compared. The results showed that sAAP(t) and sAAP(1) demonstrated better effect. In vivo test, 14-day-old chickens were injected respectively with sAAP(t), sAAP(1), AAP(t) and AAP(1) at the first vaccination of ND vaccine, once a day for three days. On days 7, 14, 21 and 28 after the first vaccination, the peripheral lymphocytes proliferation and antibody titer were determined. The results indicated that sAAP(t) possessed the best efficacy and would be expected to be used as a component of a new-type immunopotentiator. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1117 / 1122

页数：6

共 36 条

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