Generation of hapten-specific recombinant antibodies: antibody phage display technology: a review

被引：28

作者：

Brichta, J ^{[1
]}

Hnilova, M ^{[1
]}

Viskovic, T ^{[1
]}

机构：

[1] Vet Res Inst, Dept Biotechnol, Hudcova 70, CS-62132 Brno, Czech Republic

来源：

VETERINARNI MEDICINA | 2005年 / 50卷 / 06期

关键词：

antibody phage display; recombinant antibodies; hapten; antibody phage libraries; immunochemistry;

D O I：

10.17221/5620-VETMED

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Production of antibodies has been revolutionized by the development of modern molecular biology methods for the expression of recombinant DNA. Phage display technology represents one of the most powerful tools for production and selection of recombinant antibodies and has been recognized as a valuable alternative way for the preparation of antibodies of a desired specificity. In comparison to poly- and monoclonal antibodies, recombinant antibodies using the phage display technology can be prepared faster, in more automatic process and with reduced consumption of laboratory animals. This review summarizes current trends of phage display technology with focus on the generation of hapten-specific recombinant antibodies and gives the examples of successful applications of phage display in the environmental analysis of low molecular weight compound.

引用

页码：231 / 252

页数：22

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