A novel type of pyridine nucleotide-disulfide oxidoreductase is essential for NAD(+)- and NADPH-dependent degradation of epoxyalkanes by Xanthobacter strain Py2

被引：27

作者：

Swaving, J ^{[1
]}

DeBont, JAM ^{[1
]}

Westphal, A ^{[1
]}

DeKok, A ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,NL-6703 HA WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 22期

关键词：

D O I：

10.1128/jb.178.22.6644-6646.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Epoxide degradation in cell extracts of Xanthobacter strain Py2 has been reported to be dependent on NAD(+) and dithiols, This multicomponent system has now been fractionated, A key protein encoded by a DNA fragment complementing a Xanthobacter strain Py2 mutant unable to degrade epoxides was purified and analyzed. This NADP-dependent protein, a novel type of pyridine nucleotide-disulfide oxidoreductase, is essential for epoxide degradation, NADPH, acting as the physiological cofactor, replaced the dithiols in epoxide conversion.

引用

页码：6644 / 6646

页数：3

共 23 条

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