Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

被引：31

作者：

Cronin, WA

Golub, JE

Magder, LS

Baruch, NG

Lathan, MJ

Mukasa, LN

Hooper, N

Razeq, JH

Mulcahy, D

Benjamin, WH

Bishai, WR

机构：

[1] Maryland Dept Hlth & Mental Hyg, Div TB Control Refugee & Migrant Hlth, Baltimore, MD 21201 USA

[2] Maryland Dept Hlth & Mental Hyg, Labs Adm, Baltimore, MD 21201 USA

[3] Univ Maryland, Dept Epidemiol & Prevent Med, Baltimore, MD 21201 USA

[4] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Int Hlth, Baltimore, MD 21205 USA

[5] Bur Clin Labs, Dept Publ Hlth, Resp Dis Div, Montgomery, AL 36130 USA

[6] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期

关键词：

D O I：

10.1128/JCM.39.10.3709-3711.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (less than or equal to5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.

引用

页码：3709 / 3711

页数：3

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