Evidences for ubiquitination and intracellular trafficking of LAT, the linker of activated T cells

被引：24

作者：

Brignatz, C ^{[1
]}

Restouin, A ^{[1
]}

Bonello, G ^{[1
]}

Olive, D ^{[1
]}

Collette, Y ^{[1
]}

机构：

[1] Inst Cancerol, UMR599, F-13009 Marseille, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2005年 / 1746卷 / 02期

关键词：

linker of activated T cell; ubiquitination; adapter protein complex; T cell signalling; intracellular trafficking;

D O I：

10.1016/j.bbamcr.2005.08.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Current evidences indicate that T cells use protein sorting and degradation to control duration and specificity of T cell receptor (TcR) signalling, including the CD3 zeta chain which is ubiquitinated upon TcR triggering. In a previous study, we showed that the Linker of activated T cells (LAT) is present at the plasma membrane and in transferrin-labelled intracellular compartments also containing the CD3 zeta chain. Here we show that LAT protein levels are tightly regulated in Jurkat lymphoid T cells likely involving proteasome-dependent degradation, recycling through trans-Golgi/ endosome compartments and clathrin-dependent internalisation. We further identify a novel post-translational modification of LAT by ubiquitination that is likely to influence LAT protein stability, intracellular localisation and/or recycling. Our results provide novel molecular and regulatory insights into the function of LAT adapter protein in T cell signalling. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：108 / 115

页数：8

共 28 条

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