Functional cell models of the gut and their applications in food microbiology A review

被引:118
作者
Cencic, Avrelija [1 ,2 ]
Langerholc, Tomaz [2 ]
机构
[1] Univ Maribor, Fac Med, Dept Biochem, SLO-2000 Maribor, Slovenia
[2] Univ Maribor, Fac Agr & Life Sci, Dept Microbiol Biochem Mol Biol & Biotechnol, SLO-2000 Maribor, Slovenia
关键词
Cell model; Gut; Probiotics; Intestine; Pathogens; Risk assessment; TRANSEPITHELIAL ELECTRICAL-RESISTANCE; INTESTINAL EPITHELIAL-CELLS; IN-VITRO; BIFIDOBACTERIUM STRAINS; COMPETITIVE-EXCLUSION; PROBIOTIC COMBINATION; ALKALINE-PHOSPHATASE; GENE-EXPRESSION; CACO-2; CELLS; ADHESION;
D O I
10.1016/j.ijfoodmicro.2010.03.026
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Animal experimentation has a long tradition for risk assessment of new drugs before they reach the clinic. To reduce expensive animal experimentation, attempts have been made to build inexpensive and convenient intestinal functional cell models to study toxicity and bioavailability of new substances along with providing relevant models to study interactions between the host, pathogens and intestinal microflora. We review the available cell lines and models of the intestine and their potential uses. Tumor derived cell lines such as Caco-2, T84 and HT-29 are widely used despite many drawbacks, which are discussed with respect to complexity of the gut, where various cell types interact with commensal microbiota and gut-associated lymphoid tissue. To address this complexity, 3D models of human and animal gut represent a promising in vitro system to mimic in vivo situation without the use of transformed cell lines. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:S4 / S14
页数:11
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