Real-time and quantitative PCR: Applications to mechanism-based toxicology

被引：111

作者：

Walker, NJ ^{[1
]}

机构：

[1] NIEHS, Mol Epidemiol & Dosimetry Sect, Lab Computat Biol & Risk Anal, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2001年 / 15卷 / 03期

关键词：

RT-PCR; toxicogenomics; gene expression profiling; molecular epidemiology; biological models; genetic techniques; microarrays; dose-response relationships; pharmacokinetics; DNA polymorphisms;

D O I：

10.1002/jbt.8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is increasing awareness that quantitative analysis of changes in molecular targets plays a key role in addressing scientific questions in molecular toxicology, molecular epidemiology, and human risk assessment. One of the emerging technologies that is being used to analyze these molecular targets is realtime and quantitative (RTAQ) polymerase chain reaction (PCR). The aim of this review is to provide the reader with an overview of this technology and to highlight specific applications of this technology to some key areas of molecular toxicology. (C) 2001 John Wiley & Sons, Inc.

引用

页码：121 / 127

页数：7

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