Chromaffin cell survival from both young and old donors is enhanced by co-grafts of polymer-encapsulated human NGF-secreting cells

被引：16

作者：

Date, I ^{[1
]}

Ohmoto, T ^{[1
]}

Imaoka, T ^{[1
]}

Shingo, T ^{[1
]}

Emerich, DF ^{[1
]}

机构：

[1] CYTOTHERAPEUT INC,PROVIDENCE,RI 02906

来源：

NEUROREPORT | 1996年 / 7卷 / 11期

关键词：

neural transplantation; polymer; chromaffin cell; nerve growth factor; fibroblast;

D O I：

10.1097/00001756-199607290-00025

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

FOLLOWING polymer-encapsulation, human nerve growth factor-secreting baby hamster kidney fibroblasts (BHK-hNGF) were implanted into the striatum of hemiparkinsonian rats together with unencapsulated adrenal medullary chromaffin cells from either young (2 weeks) or old (12 months) donor rats. Animals receiving both BHK-hNGF cells and chromaffin cells exhibited significant decreases (39-56%) in apomorphine-induced rotational behaviour which was equivalent regardless of the age of the donor tissue. Histological analysis revealed that while survival of chromaffin cells without hNGF support was poor, co-grafts of adrenal medulla and BHK/hNGF cells increased chromaffin cell survival by 20 times. Again, this effect was independent of the age of the donor tissue. Retrieved capsules contained numerous viable encapsulated BHK-hNGF cells which continued to release hNGF. These results further indicate the potential use of intrastriatal implantation of encapsulated hNGF-secreting cells for augmenting the survival of co-grafted chromaffin cells as well as promoting the functional recovery of hemiparkinsonian rats.

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收藏

页码：1813 / 1818

页数：6

相关论文

共 24 条

[1] ADRENAL-MEDULLA GRAFTS ENHANCE RECOVERY OF STRIATAL DOPAMINERGIC FIBERS [J].

BOHN, MC ;

CUPIT, L ;

MARCIANO, F ;

GASH, DM .

SCIENCE, 1987, 237 (4817) :913-916

[2] THE USE OF GENETICALLY ALTERED ASTROCYTES TO PROVIDE NERVE GROWTH-FACTOR TO ADRENAL CHROMAFFIN CELLS GRAFTED INTO THE STRIATUM [J].

CUNNINGHAM, LA ;

HANSEN, JT ;

SHORT, MP ;

BOHN, MC .

BRAIN RESEARCH, 1991, 561 (02) :192-202

[3] Chromaffin cell survival and host dopaminergic fiber recovery in a patient with Parkinson's disease treated by cografts of adrenal medulla and pretransected peripheral nerve - Case report [J].

Date, I ;

Imaoka, T ;

Miyoshi, Y ;

Ono, T ;

Asari, S ;

Ohmoto, T .

JOURNAL OF NEUROSURGERY, 1996, 84 (04) :685-689

[4] EFFECT OF HOST AGE UPON THE DEGREE OF NIGROSTRIATAL DOPAMINERGIC SYSTEM RECOVERY FOLLOWING COGRAFTS OF ADRENAL-MEDULLA AND PRETRANSECTED PERIPHERAL-NERVE [J].

DATE, I ;

YOSHIMOTO, Y ;

IMAOKA, T ;

MIYOSHI, Y ;

FURUTA, T ;

ASARI, S ;

OHMOTO, T .

BRAIN RESEARCH, 1994, 637 (1-2) :50-56

[5] EXOGENOUS GM1 GANGLIOSIDES INDUCE PARTIAL RECOVERY OF THE NIGROSTRIATAL DOPAMINERGIC SYSTEM IN MPTP-TREATED YOUNG MICE BUT NOT IN AGING MICE [J].

DATE, I ;

FELTEN, SY ;

FELTEN, DL .

NEUROSCIENCE LETTERS, 1989, 106 (03) :282-286

[6] THE NIGROSTRIATAL DOPAMINERGIC SYSTEM IN MPTP-TREATED MICE SHOWS MORE PROMINENT RECOVERY BY SYNGENEIC ADRENAL-MEDULLARY GRAFT THAN BY ALLOGENEIC OR XENOGENEIC GRAFT [J].

DATE, I ;

FELTEN, SY ;

FELTEN, DL .

BRAIN RESEARCH, 1991, 545 (1-2) :191-198

[7] THE INFLUENCE OF DONOR AGE ON COGRAFTING OF ADRENAL-MEDULLA WITH PRETRANSECTED PERIPHERAL-NERVE [J].

DATE, I ;

YOSHIMOTO, Y ;

MIYOSHI, Y ;

IMAOKA, T ;

FURUTA, T ;

ASARI, S ;

OHMOTO, T .

BRAIN RESEARCH, 1993, 624 (1-2) :233-238

[8] COGRAFTS OF ADRENAL-MEDULLA WITH PERIPHERAL-NERVE ENHANCE THE SURVIVABILITY OF TRANSPLANTED ADRENAL CHROMAFFIN CELLS AND RECOVERY OF THE HOST NIGROSTRIATAL DOPAMINERGIC SYSTEM IN MPTP-TREATED YOUNG-ADULT MICE [J].

DATE, I ;

FELTEN, SY ;

FELTEN, DL .

BRAIN RESEARCH, 1990, 537 (1-2) :33-39

[9] EFFICACY OF PRETRANSECTION OF PERIPHERAL-NERVE FOR PROMOTING THE SURVIVAL OF COGRAFTED CHROMAFFIN CELLS AND RECOVERY OF HOST DOPAMINERGIC FIBERS IN ANIMAL-MODELS OF PARKINSONS-DISEASE [J].

DATE, I ;

MIYOSHI, Y ;

IMAOKA, T ;

FURUTA, T ;

ASARI, S ;

OHMOTO, T .

NEUROSCIENCE RESEARCH, 1994, 20 (03) :213-221

[10] ENHANCED RECOVERY OF THE NIGROSTRIATAL DOPAMINERGIC SYSTEM IN MPTP-TREATED MICE FOLLOWING INTRASTRIATAL INJECTION OF BASIC FIBROBLAST GROWTH-FACTOR IN RELATION TO AGING [J].

DATE, I ;

YOSHIMOTO, Y ;

IMAOKA, T ;

MIYOSHI, Y ;

GOHDA, Y ;

FURUTA, T ;

ASARI, S ;

OHMOTO, T .

BRAIN RESEARCH, 1993, 621 (01) :150-154