Circadian modulation of membrane properties in slices of rat suprachiasmatic nucleus

被引：73

作者：

de Jeu, M ^{[1
]}

Hermes, N ^{[1
]}

Pennartz, C ^{[1
]}

机构：

[1] Netherlands Inst Brain Res, Grad Sch Neurosci Amsterdam, NL-1105 AZ Amsterdam, Netherlands

来源：

NEUROREPORT | 1998年 / 9卷 / 16期

关键词：

amphotericin B; circadian rhythm; gramicidin; membrane potential; membrane resistance; perforated patch; spontaneous firing; suprachiasmatic nucleus;

D O I：

10.1097/00001756-199811160-00028

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

PERFORATED patch clamp recordings of neurons in slices of the suprachiasmatic nucleus (SCN) were made in their subjective day and night phases. The spontaneous firing rate and input resistance were significantly higher during the subjective day as compared to the subjective night. Ln parallel, the membrane potential of neurons recorded at daytime was significantly more depolarized than at nighttime. The day-night differences in membrane potential and input resistance were maintained when spikes and GABAA receptor-mediated transmission were blocked by tetrodotoxin and bicuculline, suggesting a cell-autonomous regulation of the circadian modulation of membrane properties. In conclusion, these findings suggest that at least one hyperpolarizing ionic conductance would be open at night and closed during mid-day, when spontaneous firing reaches peak levels in the SCN. NeuroReport 9: 3725-3729 (C) 1998 Lippincott Williams & Wilkins.

引用

页码：3725 / 3729

页数：5

共 20 条

[1]

ARMSTRONG CM, 1992, METHOD ENZYMOL, V207, P100

[2] Functional characterization of the H-current in SCN neurons in subjective day and night: a whole-cell patch-clamp study in acutely prepared brain slices [J].

deJeu, MTG ;

Pennartz, CMA .

BRAIN RESEARCH, 1997, 767 (01) :72-80

[3] CIRCADIAN-RHYTHMS OF VASOPRESSIN RELEASE FROM INDIVIDUAL RAT SUPRACHIASMATIC EXPLANTS INVITRO [J].

EARNEST, DJ ;

SLADEK, C .

BRAIN RESEARCH, 1986, 382 (01) :129-133

[4] CIRCADIAN-RHYTHM OF FIRING RATE RECORDED FROM SINGLE CELLS IN THE RAT SUPRACHIASMATIC BRAIN SLICE [J].

GREEN, DJ ;

GILLETTE, R .

BRAIN RESEARCH, 1982, 245 (01) :198-200

[5] PERSISTENCE OF CIRCADIAN RHYTHMICITY IN A MAMMALIAN HYPOTHALAMIC ISLAND CONTAINING THE SUPRACHIASMATIC NUCLEUS [J].

INOUYE, ST ;

KAWAMURA, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (11) :5962-5966

[6] Membrane properties and synaptic inputs of suprachiasmatic nucleus neurons in rat brain slices [J].

Jiang, ZG ;

Yang, YQ ;

Liu, ZP ;

Allen, CN .

JOURNAL OF PHYSIOLOGY-LONDON, 1997, 499 (01) :141-159

[7] Tracer and electrical coupling of rat suprachiasmatic nucleus neurons [J].

Jiang, ZG ;

Yang, YQ ;

Allen, CN .

NEUROSCIENCE, 1997, 77 (04) :1059-1066

[8] MEMBRANE-PROPERTIES OF RAT SUPRACHIASMATIC NUCLEUS NEURONS RECEIVING OPTIC-NERVE INPUT [J].

KIM, YI ;

DUDEK, FE .

JOURNAL OF PHYSIOLOGY-LONDON, 1993, 464 :229-243

[9] PERFORATED-PATCH RECORDING WITH GRAMICIDIN AVOIDS ARTIFACTUAL CHANGES IN INTRACELLULAR CHLORIDE CONCENTRATION [J].

KYROZIS, A ;

REICHLING, DB .

JOURNAL OF NEUROSCIENCE METHODS, 1995, 57 (01) :27-35

[10] Modalities of distortion of physiological voltage signals by patch-clamp amplifiers: A modeling study [J].

Magistretti, J ;

Mantegazza, M ;

de Curtis, M ;

Wanke, E .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :831-842

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