Alginate gel culture allows the retention of extracellular matrix and follicular structure of rat thyroid tissue but does not lead to the formation of follicles by FRTL-5 cells

被引:12
作者
Bürgi-Saville, ME
Reut, B
Gerber, H
Peter, HJ
Paulsson, M
Kaempf, J
Simon, F
Marti, U
Gerber, H
Bürgi, U [1 ]
机构
[1] Univ Hosp Bern, Dept Internal Med, Endocrine & Diabet Div, CH-3010 Bern, Switzerland
[2] Univ Hosp Bern, Cent Chem Lab, CH-3010 Bern, Switzerland
[3] Univ Hosp Bern, Div Med, Anna Seiler Haus, CH-3010 Bern, Switzerland
[4] Univ Hosp Bern, Dept Pathol, CH-3010 Bern, Switzerland
[5] Univ Cologne, Fac Med, Inst Biochem, D-5000 Cologne 41, Germany
关键词
D O I
10.1089/thy.1998.8.1147
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Extracellular matrix (ECM) and basement membrane (BM) components were studied by immunohistological methods in native rat thyroid tissue, and in rat thyroid tissue and FRTL-5 cells cultured in a three-dimensional alginate bead system. In all three situations, the presence of collagen IV, laminin, perlecan, and fibronectin was demonstrated. There were marked differences between rat thyroid tissue and FRTL-5 cells in culture. Rat thyroid tissue maintained a follicular structure, whereas FRTL-5 cells did not form follicles. Rat thyroid cells multiplied more slowly than FRTL-5 cells and thyroglobulin (Tg) was visible in the follicular lumen, while in FRTL-5 cells Tg was only seen intracellularly. Tg iodination was much lower in FRTL-5 cells than in rat cells. In rat thyroid cells, positive staining for collagen TV, laminin, and perlecan was seen in thin membranes around individual follicles, and for fibronectin around groups of follicles. In FRTL-5 cells, these ECM/BM components could be identified, but were not organized into equally regular networks around groups of cells. These results demonstrate that of the two types of cells examined, primary cultures of rat thyroid cells in alginate beads maintain structural and functional similarities to native thyroid tissue and would therefore be suitable for future irt vitro studies of thyroidal ECM/BM and their interrelationship with growth and function of this organ. FRTL-5 cells cultured in alginate beads show some functional, but not structural similarities to native thyroid tissue and so would be less valuable for use in such studies.
引用
收藏
页码:1147 / 1155
页数:9
相关论文
共 30 条
[1]  
AMBESIIMPIOMBATO FS, 1989, INT CONGR SER, V818, P3
[2]   CULTURE OF HORMONE-DEPENDENT FUNCTIONAL EPITHELIAL-CELLS FROM RAT THYROIDS [J].
AMBESIIMPIOMBATO, FS ;
PARKS, LAM ;
COON, HG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3455-3459
[3]  
ANDRE F, 1994, J CELL SCI, V107, P183
[4]  
BIDEY SP, 1989, INT CONGR SER, V818, P13
[5]   A NEW NOMENCLATURE FOR THE LAMININS [J].
BURGESON, RE ;
CHIQUET, M ;
DEUTZMANN, R ;
EKBLOM, P ;
ENGEL, J ;
KLEINMAN, H ;
MARTIN, GR ;
MENEGUZZI, G ;
PAULSSON, M ;
SANES, J ;
TIMPL, R ;
TRYGGVASON, K ;
YAMADA, Y ;
YURCHENCO, PD .
MATRIX BIOLOGY, 1994, 14 (03) :209-211
[6]   Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue [J].
BurgiSaville, ME ;
Gerber, H ;
Peter, HJ ;
Paulsson, M ;
Aeschlimann, D ;
Glaser, C ;
Kaempf, J ;
Ruchti, C ;
Sidiropoulos, I ;
Burgi, U .
THYROID, 1997, 7 (03) :347-356
[7]   PHYSIOLOGICAL AND PATHOLOGICAL REGULATION OF THYROID-CELL PROLIFERATION AND DIFFERENTIATION BY THYROTROPIN AND OTHER FACTORS [J].
DUMONT, JE ;
LAMY, F ;
ROGER, P ;
MAENHAUT, C .
PHYSIOLOGICAL REVIEWS, 1992, 72 (03) :667-697
[8]  
EKHOLM R, 1990, THYROID GLAND, P37
[9]   SYNTHESIS OF EXTRACELLULAR-MATRIX GLYCOPROTEINS BY A DIFFERENTIATED THYROID EPITHELIAL-CELL LINE [J].
GARBI, C ;
ZURZOLO, C ;
BIFULCO, M ;
NITSCH, L .
JOURNAL OF CELLULAR PHYSIOLOGY, 1988, 135 (01) :39-46
[10]  
Gerber H, 1991, Thyroidology, V3, P115