Interaction between plasmid DNA and cationic polymers studied by surface plasmon resonance spectrometry

被引：52

作者：

Wink, T

de Beer, J

Hennink, WE

Bult, A

van Bennekom, WP

机构：

[1] Univ Utrecht, Fac Pharm, UIPS, Dept Pharmaceut Anal & Human Toxicol, NL-3508 TB Utrecht, Netherlands

[2] Univ Utrecht, Fac Pharm, UIPS, Dept Pharmaceut, NL-3508 TB Utrecht, Netherlands

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 04期

关键词：

D O I：

10.1021/ac980679d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A universal and novel strategy for the immobilization of polymers has been developed for studying the interaction between plasmid DNA and synthetic polymers with surface plasmon resonance spectrometry. The introduction of thiol moieties in polymers has been applied for a reliable determination of apparent kinetic rate constants between plasmid DNA and the nonviral carrier polymers. Thiolated poly(L-lysine) and poly[(2-dimethylamino)ethyl methacrylate] yielded reproducible sensor surfaces, contrary to the nonthiolated polymers. The knowledge of the kinetic parameters may play a crucial role in the development of nonviral carrier systems for gene therapy, because the dissociation rate constant is strongly correlated to the effectiveness of cell transfection.

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页码：801 / 805

页数：5

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