TaqMan based real time PCR method for quantitative detection of basmati rice adulteration with non-basmati rice

被引：22

作者：

Lopez, S. Joseph ^{[1
]}

机构：

[1] SGS India Private Ltd, Dept Biotechnol, Ahmadabad 380055, Gujarat, India

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2008年 / 227卷 / 02期

关键词：

basmati; real time PCR; betaine aldehyde dehydrogenase;

D O I：

10.1007/s00217-007-0763-0

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A TaqMan based real time PCR method is developed for the first time to check the adulteration in basmati rice. Betaine aldehyde dehydrogenase 2 (BAD2) gene specific primers and probes were used to detect adulterant non-basmati rice content in basmati samples. The results obtained with market samples and validation samples (basmati rice spiked with non-basmati rice in different proportions) clearly revealed that this technique is useful to detect the adulteration very accurately with 1% detection limit.

引用

页码：619 / 622

页数：4

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