Rapid enzyme immunoassay for measurement of bovine progesterone

被引：27

作者：

Claycomb, RW

Delwiche, MJ ^{[1
]}

Munro, CJ

BonDurant, RH

机构：

[1] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[2] DDx Inc, Denver, CO 80221 USA

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 11期

关键词：

cow; estrus; antibody; hormone;

D O I：

10.1016/S0956-5663(98)00081-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：1165 / 1171

页数：7

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