Comparative analysis of the glg operons of Pectobacterium chrysanthemi PY35 and other prokaryotes

被引：6

作者：

Cho, Kye Man ^{[1
]}

Lim, Woo Jin ^{[1
]}

Math, Renukaradhya K. ^{[1
]}

Islam, Shah Md. Asraful ^{[1
]}

Hong, Sun Joo ^{[1
]}

Kim, Hoon ^{[2
]}

Yun, Han Dae ^{[1
,3
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci, BK21 Program, Grad Sch, Chinju 660701, South Korea

[2] Sunchon Natl Univ, Dept Agr Chem, Sunchon 540742, South Korea

[3] Gyeongsang Natl Univ, Res Inst Agr & Life Sci, Chinju 660701, South Korea

来源：

JOURNAL OF MOLECULAR EVOLUTION | 2008年 / 67卷 / 01期

关键词：

Pectobacterium chrysanthemi; PY35 glg operon; gene organization; glycogen metabolism; phylogeny; prokaryotes;

D O I：

10.1007/s00239-008-9103-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chromosomal region of Pectobacterium chrysanthemi PY35 that contains of genes for glycogen synthesis was isolated from a cosmid library. The operon consists of glycogen branching enzyme (glgB), glycogen debranching enzyme (glgX), ADP-glucose pyrophosphorylase (glgC), glycogen synthase (glgA), and glycogen phosphorylase (glgP) genes. Gene organization is similar to that of Escherichia coli. The purified ADP-glucose pyrophosphorylase (GlgC) was activated by fructose 1,6-bisphosphate and inhibited by AMP. The constructed glgX::Omega mutant failed to integrate into the chromosome of P. chrysanthemi by marker exchange. Phylogenetic analysis based on the 16S rDNA and the amino acid sequence of Glg enzymes showed correlation with other bacteria. gamma-Proteobacteria have the glgX gene instead of the bacilli glgD gene in the glg operon. The possible evolutionary implications of the results among the prokaryotes are discussed.

引用

页码：1 / 12

页数：12

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