Human Rad50/Mre11 is a flexible complex that can tether DNA ends

被引：384

作者：

de Jager, M

van Noort, J

van Gent, DC

Dekker, C

Kanaar, R

Wyman, C

机构：

[1] Erasmus Univ, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

[2] Delft Univ Technol, Dept Appl Phys, NL-2628 CJ Delft, Netherlands

[3] Delft Univ Technol, DIMES, NL-2628 CJ Delft, Netherlands

[4] Univ Rotterdam Hosp, Dept Radiat Oncol, Rotterdam, Netherlands

来源：

MOLECULAR CELL | 2001年 / 8卷 / 05期

关键词：

D O I：

10.1016/S1097-2765(01)00381-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human Rad50 protein, classified as a structural maintenance of chromosomes (SMC) family member, is complexed with Mre11 (R/M) and has important functions in at least two distinct double-strand break repair pathways. To find out what the common function of R/M in these pathways might be, we investigated its architecture. Scanning force microscopy showed that the complex architecture is distinct from the described SMC family members. R/M consisted of two highly flexible intramolecular coiled coils emanating from a central globular DNA binding domain. DNA end-bound R/M oligomers could tether linear DNA molecules. These observations suggest that a unified role for R/M in multiple aspects of DNA repair and chromosome metabolism is to provide a flexible, possibly dynamic, link between DNA ends.

引用

页码：1129 / 1135

页数：7

共 38 条

[1] Structure of the Rad50-Mre11 DNA repair complex from Saccharomyces cerevisiae by electron microscopy [J].