In vitro phonophoresis of mannitol, oestradiol and hydrocortisone across human and hairless mouse skin

被引:14
作者
Machet, L [1 ]
Cochelin, N
Patat, F
Arbeille, B
Machet, MC
Lorette, G
Vaillant, L
机构
[1] CHU Trousseau, Serv Dermatol, F-37044 Tours, France
[2] Fac Med Tours, EA 2102, Lab UltraSons Signaux & Instrumentat, F-37032 Tours, France
[3] Fac Med Tours, Lab Microscopie Elect, F-37032 Tours, France
[4] CHU Trousseau, Serv Anat Pathol, F-37044 Tours, France
关键词
percutaneous administration; ultrasound; human skin; hairless mouse skin; phonophoresis; electron microscopy;
D O I
10.1016/S0378-5173(97)00377-3
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Ultrasound is frequently used in physical medicine. In a preliminary study, we demonstrated that the thermal effect of ultrasound was the principal explanation for the increase in the diffusion rate of digoxin. The aim of this study was to investigate further the phonophoresis of three drugs with a cooling thermostat, thus suppressing the thermal effect of ultrasound. Sonication was carried out at 1.5 W/cm(2), 1.1 MHz for 20 min. We used modified Franz diffusion cells adapted for sonication. During sonication the temperature in the donor compartment was continuously monitored and maintained at 31 degrees C by the cooling coil. Diffusion of the tritiated drugs (hydrocortisone, mannitol, oestradiol) across hairless mouse and whole human skin was determined by liquid scintillation counting for up to 24 h, and the steady-state flux was determined. No enhancement in steady-state diffusion rates was observed for the three drugs in comparison with controls. The thermal effect of ultrasound seems to be the main factor which enhances percutaneous administration under the conditions used in physical medicine. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:169 / 174
页数:6
相关论文
共 13 条
[1]   USE OF ULTRASOUND TO ENHANCE PERCUTANEOUS-ABSORPTION OF BENZYDAMINE [J].
BENSON, HAE ;
MCELNAY, JC ;
HARLAND, R .
PHYSICAL THERAPY, 1989, 69 (02) :113-118
[2]   SONOPHORESIS .1. THE USE OF HIGH-FREQUENCY ULTRASOUND TO ENHANCE TRANSDERMAL DRUG DELIVERY [J].
BOMMANNAN, D ;
OKUYAMA, H ;
STAUFFER, P ;
GUY, RH .
PHARMACEUTICAL RESEARCH, 1992, 9 (04) :559-564
[3]   METHODS FOR INVITRO PERCUTANEOUS-ABSORPTION STUDIES .7. USE OF EXCISED HUMAN-SKIN [J].
BRONAUGH, RL ;
STEWART, RF ;
SIMON, M .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1986, 75 (11) :1094-1097
[4]   THE EFFECT OF ULTRASOUND ON THE INVITRO PENETRATION OF IBUPROFEN THROUGH HUMAN-EPIDERMIS [J].
BRUCKS, R ;
NANAVATY, M ;
JUNG, D ;
SIEGEL, F .
PHARMACEUTICAL RESEARCH, 1989, 6 (08) :697-701
[5]   THE USE OF ULTRASOUND AS AN ENHANCER FOR TRANSCUTANEOUS DRUG-DELIVERY - PHONOPHORESIS [J].
BYL, NN .
PHYSICAL THERAPY, 1995, 75 (06) :539-553
[6]   In vitro phonophoresis of digoxin across hairless mice and human skin: Thermal effect of ultrasound [J].
Machet, L ;
Pinton, J ;
Patat, F ;
Arbeille, B ;
Pourcelot, L ;
Vaillant, L .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1996, 133 (1-2) :39-45
[7]   HIGH-FREQUENCY SONOPHORESIS - PERMEATION PATHWAYS AND STRUCTURAL BASIS FOR ENHANCED PERMEABILITY [J].
MENON, GK ;
BOMMANNAN, DB ;
ELIAS, PM .
SKIN PHARMACOLOGY, 1994, 7 (03) :130-139
[8]   Transdermal drug delivery using low-frequency sonophoresis [J].
Mitragotri, S ;
Blankschtein, D ;
Langer, R .
PHARMACEUTICAL RESEARCH, 1996, 13 (03) :411-420
[9]   MECHANISTIC STUDY OF ULTRASONICALLY-ENHANCED TRANSDERMAL DRUG-DELIVERY [J].
MITRAGOTRI, S ;
EDWARDS, DA ;
BLANKSCHTEIN, D ;
LANGER, R .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1995, 84 (06) :697-706
[10]  
PELUCIOLOPES C, 1993, INT J PHARM, V90, P1