Replication in UV-irradiated Caenorhabditis elegans embryos

被引:15
作者
Jones, CA [1 ]
Hartman, PS [1 ]
机构
[1] TEXAS CHRISTIAN UNIV, DEPT BIOL, FT WORTH, TX 76129 USA
关键词
D O I
10.1111/j.1751-1097.1996.tb03012.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Replication continues in wild-type (but not rad mutant) Caenorhabditis elegans embryos even after exposure to massive fluences of UV radiation. It is of interest to elucidate the mechanism(s) for this ''damage-resistant'' DNA synthesis. In this study, DNA from unirradiated and UV-irradiated wild-type embryos was examined using the electron microscope. Large fluences of UV radiation (180 J m(-2)) had little effect on either replication bubble size or distances between bubbles in wild-type embryos, indicating that the damage-resistant DNA synthesis was not grossly aberrant. Conversely, UV irradiation significantly decreased center-to-center distances between bubbles in excision-repair-deficient rad-3 embryos. This suggests that the decreased DNA synthesis observed after UV irradiation in rad-3 embryos is due largely to blockage of elongation of DNA synthesis.
引用
收藏
页码:187 / 192
页数:6
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