Heteronuclear correlation experiments for the determination of one-bond coupling constants

被引：30

作者：

Andersson, P

Nordstrand, K

Sunnerhagen, M

Liepinsh, E

Turovskis, I

Otting, G ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[2] Inst Organ Synth, LV-1006 Riga, Latvia

来源：

JOURNAL OF BIOMOLECULAR NMR | 1998年 / 11卷 / 04期

关键词：

E-coli arginine repressor; HMQC-alpha/beta; HSQC-alpha/beta; one-bond coupling;

D O I：

10.1023/A:1008206212145

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/beta, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, H-1-detected N-15-H-1 or C-13-H-1 correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.

引用

页码：445 / 450

页数：6

共 38 条

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