Purification of ADAM 10 from bovine spleen as a TNF alpha convertase

被引：113

作者：

Lunn, CA

Fan, XD

Dalie, B

Miller, K

Zavodny, PJ

Narula, SK

Lundell, D

机构：

[1] Department of Immunology, Schering-Plough Research Institute, Kenilworth

来源：

FEBS LETTERS | 1997年 / 400卷 / 03期

关键词：

tissue necrosis factor alpha; TNF alpha convertase; ADAM; 10; metalloprotease;

D O I：

10.1016/S0014-5793(96)01410-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified a protease with characteristics of TNF alpha convertase from bovine spleen membranes. Peptide sequencing of the purified protein identified it as ADAM 10 (Genbank accession no. Z21961). This metalloprotease cleaves a recombinant proTNF alpha substrate to mature TNF alpha, and can cleave a synthetic peptide substrate to yield the mature TNF alpha amino terminus in vitro. The enzyme is sensitive to a hydroxamate inhibitor of MMPs, but insensitive to phosphoramidon. In addition, cloned ADAM 10 mediates proTNF alpha processing in a processing-incompetent cell line.

引用

页码：333 / 335

页数：3

共 13 条

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